Antimicrobial activity and phytochemical analysis of Rivea ornate
Karunarathna A.*1, Jeyadevan J.1 and Thavaranjit A.2
Keywords:Rivea ornate, antibacterial, antifungal, Phytochemical analysis
Rivea ornate is an important medicinal plant in the family Convolvulceae. Extracts of young leaves, matured leaves, stem and roots of Rivea ornata were obtained by extraction method using hexane and methanol. Antifungal, antibacterial assay, antibiotic resistance and preliminary phytochemical analysis were carried out. Antibacterial activity was determined against Pseudomonas sp., Proteus sp., Enterococcus sp., Staphylococcus sp. and E, coli and antifungal activity was determined against Mucor sp., Rhizopus sp. and Aspergillus sp. by using the standard agar well diffusion method. Antibiotic resistance of tested bacteria was determined by the disc method against bacitracin, gentamycin, amoxycilin and streptomycin. The standard Mancozeb had no effect on Rhizopus sp. Only methanol extract of young leaf showed the higher degree of antifungal activity against Mucor sp. and Aspergillus sp. Methanol extract of stem inhibited the growth of Rhizopus sp. predominantly.
Hexane and methanol extracts of stem showed activity only against Enterococcus sp. and E. coli respectively. Methanol extract of root only exhibited activity against Enterococcus sp. Methanol extract of young leaf had the predominant antibacterial activity among the tested different extracts against the bacterial species. This study also revealed that E.coli and Pseudomonas sp. had the multi resistance ability for antibiotics. Phytochemical analysis of methanolic extract of aerial parts revealed that the presence of alkaloids, saponins, tannins, coumarins and Cardiac Glycosides. The antimicrobial activity of Methanolic extracts of young leaves, and matured leaves of Rivea ornata is due to the presence of bioactive compounds.